To monitor the delivery of EVs to target cells in vivo, we make use of a reporter strategy.We utilize transgenic mouse models (reporters) to determine EV-mediated neural cell communication and its prevalence in distinct areas of the brain. The strategy involves oligodendroglial CreERT2 drivers crossed to Rosa26-loxP reporter mice. Transfer of CreERT2 to neurons results in neuronal reporter gene activation, which can be quantified in brain slices within different brain regions. We further confirm neuronal target cell recombination due to EV-transfer by genetically interfering with EV-biogenesis and release in these reporter mice.
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. Frühbeis C, Fröhlich D, Kuo WP, Amphornrat J, Thilemann S, Saab AS, Kirchhoff F, Möbius W, Goebbels S, Nave KA, Schneider A, Simons M, Klugmann M, Trotter J, Krämer-Albers EM. PLoS Biol. 2013 Jul;11(7):e1001604. doi: 10.1371/journal.pbio.1001604. Epub 2013 Jul 9. PMID: 23874151 Free PMC article.
Laura Stopper (Homburg Saar)
Former Lab Members